血吸虫肝卵免疫酶试验切片载体的改进

AN IMPROVEMENT OF LIVER EGG-IMMUNOENZYMATIC STAINING TECHNIQUE FOR DIAGNOSIS OF SCHISTOSOMIASIS JAPONICA

应用PVC薄膜代替载玻片作肝卵组织切片载体,进行免疫酶染色试验(简称PVC-IEST)。与玻片法免疫酶染色试验(IEST)比较,两法同时检测78份血吸虫病患者血清,结果PVC-IEST和IEST阳性率分别为96.2％和97.4％。此外,用PVC-IEST检测粪便孵化阳性的血吸虫病患者血清164份和粪便孵化阴性而COPT阳性的血清15份,其PVC-IEST阳性率为97.2％(174/179)。检测6份并殖吸虫病患者血清及并殖吸虫病犬血清6份,均为阴性。健康人血清63份,62份阴性,仅1例假阳性。

Using PVC membrane instead of common slide in liver egg-immunoenzymatic staining technique (IEST) for diagnosis of schistosomiasis japonica to detect specific antibodies in sera from 179 cases with schistosomiasis japonica,the positive rate was 97.2% (174/ 179).Sera from 63 healthy individuals revealed in one positive (1.5%).No cross reaction was observed in sera from 6 cases of paragonimiasis.PVC-IEST and SLIDE-IEST were comparatively performed on sera from 78 cases with schistosomiasis japonica,the positive rates were 96.2% (75/78) and 97.4% (76/78),respectively (P>0.05).As PVC-IEST is simpler to perform than SLIDE-IEST,it is suggested that the former is more suitable for application in the field for diagnostic purposes.