日本血吸虫虫卵肉芽肿内抗原和抗体的动态

THE DYNAMICS OF ANTIGEN AND ANTIBODY IN SCHISTOSOMA JAPONICUM EGG GRANULOMA AND ITS RELATIONSHIP WITH THE GRANULOMA RESPONSE

用日本血吸虫虫卵注入肺内同步产生肉芽肿的小鼠肺模型进行研究。发现新鲜虫卵注入未致敏小鼠肺内,3.5d即出现细胞反应,28d达高峰,淋巴细胞和巨噬细胞是早期的主要成分。虫卵内抗原(SEA)于注射后第1d最高,随即逐渐下降,卵周抗原3.5d开始逐渐增高,28d达到高峰,其后逐渐减少,未测到抗-SEA抗体。以抗原消耗的虫卵注入致敏小鼠,至第35d实验结束时未见明显肉芽肿形成。结果表明新鲜虫卵能对未致敏小鼠诱发肉芽肿形成;日本血吸虫虫卵肉芽肿形成的机制与曼氏血吸虫者相似,也是虫卵抗原(SEA)诱发的细胞免疫为主的超敏反应。

Synchronous Schistosoma japonicum egg granuloma in the lung of mouse model and PAP (peroxidas-anti-peroxidase) technique were employed to study the dynamics of antigen and antibody in the egg granuloma of S.japouicum and its relationship with the granuloma formation.The granulomatous response began on the 3.5 day after egg injection and increased to the maximal size at the 4th week.Lymphocyte populations and macrophage comprised an important part of lesions during the time of acute granulomatous response (7-28 day).Using PAP staining,the SEA within egg could be detected at high level on the first day after egg injection and then declined gradually.On the contrary,the SEA around egg was minimal at the first week and increased to the peak at the 4th week,then decreased gradually.No antibody could be detected throughout the experimental period (35 days).The results suggested that 1) the antigen of Schistosoma egg is the essential factor for the granuloma formation.2) S.japonicum egg granuloma could be formed in unsensitized mouse.3) The mechanism of egg granuloma formation of S.japonicum is similar to that of S.mansoni.