免疫增强剂“425”对日本血吸虫感染小鼠虫卵肉芽肿病变作用的研究

STUDIES ON THE EFFECT OF IMMUNOENHANCING REAGENT “425” ON EGG GRANULOMA FORMATION IN MICE INFECTED WITH SCHISTOSOMA JAPONICUM

应用免疫增强剂当归根提取液,其化学成分主要含有阿魏酸,VitB_(12)和聚乙炔类化合物(代号为“425”),ip 25mg/d·14,至小鼠感染日本血吸虫后14—34d,肝组织内特异性抗体(IgG)为362.67±162.21—480.00±289.45,显著高于对照组的66.67±39.68—245.33±101.50(P<0.01或0.05)。虫卵肉芽肿病变则明显减弱,小鼠虫卵肉芽肿反应增长比为5.24±1.04—3.95±0.77,对照组为6.69±1.19—5.29±0.94。而且还使肝组织内虫卵抗原水平降低。结果表明,免疫增强剂“425”可诱发宿主产生免疫调节作用,控制虫卵肉芽肿病变。

Fourteen-34 days after Schistosoma japonzcum-infected mice were injected intraperitoneally with immunoenhancing reagent,Chinese angelica root extract ('425'),the specific antibody (IgG) levels were significantly higher in Group Ⅲ (362.67±162.21-480.00±289.45) than the control group (66.67±39.68-245.33±101.56) in experiment one,and in Gronp Ⅱ(488.00±320.38-768.00±267.38) than the control group (256.00±189.07-394.67±141.06) in experiment two.The egg granulomatous formation were markedly diminished in the injected mice.The ratios of granulomas vs.eggs' mean diameters of Group in of mice were 5.24±1.04-3.95±0.77 and those of the control group were 6.59±1.19-5.29±0.94 in experiment one,those of the group Ⅱ were 3.75±0.71-4.15±0.73 and those of the control group were 4.94±0.81-5.36±0.97 in experiment two.Meantime the egg antigen levels in the injected mice might be lower.This study shows that the control of immunomodulation of granulomatous formation in the hosts injected with immunoenhance reagent '425' can be induced.