免疫金银法标记抗猪囊尾蚴囊液抗原McAb与猪囊尾蚴特异性结合的定位研究

STUDY ON LOCALIZATION OF CYSTIC FLUID ANTIGEN OF CYSTICERCUS CELLULOSAE USING McAb-IGSS

McAb F_3在猪囊尾蚴上的结合部位经免疫金银法标记定位,冰冻及石腊切片均显示出清晰的结合位点。囊壁部位颗粒均匀呈双层分布,头节折叠部位皱襞的凹陷处黑色颗粒呈密集的小片状。免疫金银法所标记的抗猪囊尾蚴囊液与其相应抗原的结合部位与囊尾蚴细胞层的分布相一致。

A study was undertaken on the localization of specific combination of McAb against cystic fluid antigen of Cysticercus cellulosae by immunogold silver staining method (IGSS).It was shown that the combination sites of McAb F3 were distinct in the frozen and paraffin sections of Cysticercus cellulosae.The black granules in the bladder walls presented double-layer distribution and those in the scolex folded sites concentrated into pieces.The combination sites of McAb labeled by IGSS against cystic fluid antigen of Cysticercus cellulosae were consistent with the distribution of the cell layers of Cysticercus cellulosae.The sections in the control group had scarce and irregular distribution,which could be differentiated distindtly from those in the experimental group.