旋毛虫新生幼虫抗原的免疫印迹分析

ANALYSIS OF NEWBORN LARVA OF TRICHINELLA SPIRALIS BY IMMUNOBLOT

利用免疫印迹法分析了旋毛虫的新生幼虫(NBL)抗原,并与旋毛虫的成虫和肌肉期幼虫抗原加以比较。NBL虫体组分经SDS-PAGE分离出约40条区带,与成虫和肌肉期幼虫的电泳图谱明显不同。免疫印迹表明,用NBL作免疫原可诱导家兔产生具有期特异性的免疫反应,其有抗原性的分子量分别为129、120、89、87、79、72、64、58、43、40、38、34、32和20kDa。但在自然感染过程中,宿主体内未检测到抗NBL的抗体。

Newborn larva (NBL) antigens of Trichinella spiralis were analysed by Immuroblot,and were comparied with the adult and muscle larva antigens.The SDS-PAGE patterns of NBL somatic constituents consisted of about 40 polypeptide bands,which were obviously different from those of adult and muscle larva.Immunoblot analysis indicated that immunization with NBL could induce a stage specific immune response.The molecular weight of specific NBL antigens were 129,120,89,87,79,74,72,64,58,43,40,38,34,32,and 20kDa.But during the natural course of the infection,we could not detect the antibodies of anti-NBL in the host.