中国大陆日本血吸虫品系的研究——Ⅷ.感染动物血清免疫学反应

STUDIES ON THE STRAIN DIFFERENCES OF SCHISTOSOMA JAPONICUM IN THE MAINLAND OF CHINA VIII. IMMUNOREACTIONS IN EXPERIMENTAL ANIMALS

本文观察了安徽、湖北、四川、云南、广西5地日本血吸虫感染小鼠、大鼠、金黄仓鼠、兔、恒河猴等5种动物的血清对同源及异源虫卵的环卵沉淀试验(COPT)反应状况以及应用安徽虫卵制备的可溶性抗原进行胶乳凝集试验(LAT)及酶联免疫吸附试验(ELISA)检测同源或异源虫体所致的血吸虫病的抗体情况,未见应用同源虫卵抗原检测感染动物的结果显著地优于异源的。这提示在中国大陆应用异地血吸虫卵抗原进行上述血清免疫学反应检测时似不影响其阳性检出率。

Electrophoretic techniques have been used to survey five different field-collected isolates (i.e.,Anhui,Hubei,Guangxi,Sichuan and Yunnan) of S.japonicum from the mainland of China for genetic variation at 7 isozymes markers representing 9 loci.Polymorphic loci were observed for LDH-1,LDH-2,MDH and PGM,respectively,the proportion of polymorphic loci being 44.4%.Average heterozygosity varied between 0.223 and 0.425,with a mean of 0.332.Nei's genetic distance (D) among the populations of 5 isolates gave values between 0.001 and 0.039 with an average of 0.023,indicating that these isolates are very closely rehted.