日本血吸虫24-26kDa抗原和重组Sj26抗原的抗原性和免疫原性比较研究

COMPARATIVE STUDY ON ANTIGENICITY AND IMMUNOGENICITY OF THE 24-26 kDa ANTIGEN AND THE RECOMBINANT Sj26 ANTIGEN OF SCHISTOSOMA JAPONICUM

本文报告从日本血吸虫大陆株成虫抽提的24—26kDa抗原与源于日本血吸虫菲律宾株的重组Sj26抗原之间在抗原性和免疫原性上所进行的一系列比较研究。ELISA、IFA和Westernblotting等方法观察结果均表明,24—26kDa和rSj26抗原免疫后所诱导的特异性抗体与其对应的抗原之间具有明显的抗原交叉反应,而与其它血吸虫抗原如可溶性虫卵抗原也有交叉反应。若以两种抗原加福氏佐剂分别免疫小鼠,以日本血吸虫大陆株尾蚴攻击感染,减虫率相似(26～32％),表明两种抗原存在一定程度的交叉免疫保护性。这些研究结果为开发rSj26抗原,或是根据已知Sj26 cDNA核苷酸序列制备DNA探针,从已构建的日本血吸虫(大陆株)cDNA库筛选相关目的基因,加速血吸虫疫苗的研制起着重要作用。

This paper reports on the comparison of the recombinant Sj26 (rSj26) antigen originated from the Philippine strain and 24-26 kDa antigen isolated and purified from Chinese mainland strain of S.japonicum for their antigenicity and immunogenicity.The results showed that there were obvious cross reactions between rSj26 and 24-26 kDa anitigen when rSj26 antigen was tested against specific antibodies in sera from mice infected with the mainland strain of S.japonicum or 24-26 kDa antigen was tested against specific anti-rSj26 antibodies by ELISA,IFA and Western blotting etc.Both of 24-26 kDa and rSj26 antigen had weak cross reaction With SEA antigen.The worm reduction rate after challenging with mainland strain cercariae in mice immunized with rSj26 was 26~32%,similar to that in mice immunized with 24-26 kDa antigen.It is suggested that rSj26 antigen can induce certain level of specific protective immunity to protect the host against infection by Chinese strain of S.japonicum cercaciae.