巨噬细胞加工与呈递日本血吸虫抗原的作用

PRELIMINARY STUDY ON THE ROLE OF MACROPHAGE IN PROCESSING AND PRESENTING ANTIGENS OF SCHISTOSOMA JAPONICUM

以免疫印迹技术探讨Mφ对日本血吸虫UEA(卵尿素溶性抗原)的加工情况。检测对象为UEA体外激活的Mφ匀浆及Mφ培养上清。所用结合物为辣根过氧化物酶标记的经亲和层析提纯的小鼠抗UEA抗体。结果可见抗原经Mφ作用后,大分子量UEA形成小分子多肽。它们与UEA及胰酶酶解的UEA有明显差异;培养上清与细胞匀浆条带较相似,仅见某些量的不同。根据以上结果而推论:(1)UEA可被Mφ加工为小分子片段,加工方式为裂解;(2)加工后的抗原多肽可释出Mφ外。

The horseradish peroxidase labeled affinity purified mice anti-UEA (Urea soluable egg antigen of Schistosoma japonicum) antibody was used in enzyme-linked immunoe ectrotransfer blot (EITB) te monitor the changes after UEA being processed by Mφ.The immune respon-sive peptides were delected in the culture supernatant and homogenate of Mφ pulsed with UEA in vitro (Mφ+).After processing by Mφ the high molecular weight UEA was cleaved into low molecular weight peptides,as shown,by the reactive bands.They markedly differed from that native UEA or trypsin-digested UEA; The bands of Mφ supernatant and homogenate showed similarity with certain quantitative differences.According to the result described above,we considered: 1.UEA could be processed into smaller pieces by Mφ,the style of processing is cleavage.2.The processed peptides might be released to extracellular environment.