用ELISA进行丝虫病血清流行病学监测

SEROLOGICAL EPIDEMIOLOGIC SURVEILLANCE ON FILARIASIS BY ELISA

1984—1990年在马来丝虫病基本消灭地区余杭县,对6个村的人群,用马来丝虫成虫可溶性抗原的ELISA检测血清抗体,进行纵向及横向血清流行病学监测。结果表明在丝虫病基本消灭5年后,人群微丝蚴血症率下降至0,ELISA阳性率和平均消光值均与非丝虫病流行区人群的阳性率(2.53％)和平均消光值(0.17±0.13)相近。纵向监测点检测结果表明10岁以下儿童的ELISA阳性率下降最明显,在丝虫病基本消灭后第3、5年均未检出阳性反应者。同时用ELISA检测班氏丝虫病家及其周围人群的血清抗体,其阳性率为8.33％,且在10岁以下儿童测得阳性反应者。我们认为用马来丝虫成虫抗原的ELISA检测人群血清抗体是一种有效的丝虫病血清学监测方法。低年龄儿童的阳性率可反映该病在当地的传播状况。

In 1984-1990 longitudinal or sectional serological surveillance on filariasis by ELISAwith Brugia malayi adult antigen was carried out in 6 villages,Yuhang County,ZhejiangProvince,where malayan filariasis had been under control.During that period the microfila-raemia rate of inhabitants had been declining continuously till nil,the positive rate andmean optical density (OD) value in ELISA for positive inhabitants concurrently declinedyear by year,especially for children under age of 10.By the 5th year after control boththe positive rate and mean OD value were close to those of healthy persons living in no-nendemic areas of filariasis,no positivity was found in children.In the meantime,ELISAwas used to detect serum antibodies in members of a family who were infected with ban-croftian filariasis and inhabitants living around them in 1990.Positive rate of ELISA was higher in these inhabitants (8.33%) than in healthy persons (2.53%),and positive oneswere detected from children under age of 10.The results suggest shat the detection of antifilarial antibody by using ELISA is an effective method for the surveillance of filariasis.