摘要
将泡球蚴保存于-10℃130d后,再移植至小鼠腹腔内,并分别于接种后第67d和225d剖检,均见虫周围有淋巴细胞浸润和纤维组织增生,有的形成淋巴性坏死、坏死腔、钙颗粒,示泡球蚴已丧失活力;而将泡球蚴保存于-30℃200d后,在接种后第157d剖检,可见囊壁角质层、生发膜清晰,囊内有多个原头节及其小钩结构、母囊泡、子囊泡和大小不等的钙颗粒等,表明其有活力和感染力。结果提示冰冻保存离体泡球蚴的活力与温度有关,-30℃比-10℃更有利于泡球蚴的存活。
Alveolar hydatids of Echinococcus multilocularis obtained from the peritoneal cavity of mice were subject to freezing at-10℃ and-30℃ respectively.After 130-200 days' storage,the alveolar hydatids were transferred into the mouse peritoneal cavity for viability and inactivity testing.It was found that the alveolar hydatids stored at-10℃ were more severely damaged than those stored at-30℃.The viability of the isolated alveococcus lasted half a year at-30℃.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
1992年第4期307-310,共4页
Chinese Journal of Parasitology and Parasitic Diseases
关键词
泡球蚴
多房棘球绦虫
冰冻保存
Alveolar hydatid,Echinococcus multilocularis,cryopreservatioa
