中国大陆日本血吸虫品系的研究 XI.酶联免疫电转移印迹的分析

STUDIES ON THE STRAIN DIFFERENCES OF SCHISTOSOMA JAPONICUM IN THE MAINLAND OF CHINA XI. ENZYME LINKED IMMUNOELECTROTRANSFER BLOT ANALYSIS

本文报告安徽、湖北、广西、云南和四川5地日本血吸虫成虫抗原与安徽、湖北、云南、四川4地钉螺的抗血清进行酶联免疫电转移印迹(EITB)分析的结果。安徽与湖北两地日本血吸虫EITB图谱基本相似。云南和四川的日本血吸虫EITB图谱虽相近,但在84kDa处云南雌虫呈现明显条带而四川雌虫则无此反应带。广西的日本血吸虫与云南的虫体一样,雌虫在84kDa处亦呈现明显的条带。广西的雄虫在与安徽的钉螺抗血清反应后在>130kDa处有两条主要条带,这与安徽地区雄虫的一致且较之更为浓染致密,而云南、四川两地雄虫的则未见此条带。根据上述结果并结合以前有关大陆各地钉螺对日本血吸虫的易感性结果,进行了初步的分析和讨论。

Antigens prepared from S.japonicum adult worms of different isolates i.e.Anhui,Hubei,Guangxi,Yunnan and Sichuen by origin were analyzed by enzyme linked immunoe-lectrotransfer blot (EITB) probed with rabbit anti-snail antibody (Figs 1,2).Anti-sera against Oncomelania h,hupensis from Anhui,Hubei,Yunnan and Sichuan localities were prepared separately and used in the tests.The EITB patterns were similar in S.j.isolates of Anhui and Hubei,and it was also the case among S.7.isolates of Yunnan and Sichuan except Yunnan female worms with a marked band of 84 kDa but it was almost invisible in EITB pattern of Sichuan female worms.Like Yunnan isolate,female worms of Guangxi isolate also showed marked 84 kDa bands.The EITB pattern of male worms from Guangxi isolate showed 2 main bands of mw>130 kDa against anti-Anhui snail anti-serum,which corresponded with the male worms of Anhui isolate whereas the color of the bands was darker and denser in the former isolates,and these bands can not be seen in the male worms from isolates of Yunnan and Sichuan.Based on the above mentioned results in connection with the information about the susceptibility between different isolates of schistosemes and their snail hosts which we have reported before,some preliminary analysis and discussion were made.