利用套叠PCR和高保真DNA聚合酶进行基因多位点突变的研究

Multi-mutation of FMDV-VP1 Gene by Overlap Extension of PCR with High-fidelity Thermostable DNA Polymerase

利用套叠PCR和高保真DNA聚合酶对人工合成的牛口蹄疫病毒VPI基因(FMDV-VPl,Foot-Mouth Disease Virus-VPl)的5个突变位点进行修复,修复的成功率为100％。

Five mutant sites of VP1 gene synthesized artificially from cattle Foot-Mouth Disease Virus (FMDV) were rectified by overlap extension with high-fidelity thermostable DNA polymerase, the rate of success was 100 percent.