摘要
利用套叠PCR和高保真DNA聚合酶对人工合成的牛口蹄疫病毒VPI基因(FMDV-VPl,Foot-Mouth Disease Virus-VPl)的5个突变位点进行修复,修复的成功率为100%。
Five mutant sites of VP1 gene synthesized artificially from cattle Foot-Mouth Disease Virus (FMDV) were rectified by overlap extension with high-fidelity thermostable DNA polymerase, the rate of success was 100 percent.
出处
《生命科学研究》
CAS
CSCD
2004年第1期28-31,共4页
Life Science Research
基金
2002年中国热带农业科学院重点方向资助项目