摘要
根据GenBank数据库中的生物信息,利用BLAST免费分析软件找出4种型别登革病毒的保守序列及各型特异性序列,针对上述序列设计引物经RT-PCR扩增登革病毒的特异片段。利用此RT-PCR法收集探针是一种快速、简便制备基因芯片探针的实用方法。
To develope the microarray probes for detection of the 4 serotypes of dengue virus by RT-PCR. The genomes were and sequence alignment was done by using BLAST biosoftware and data from Genebank to find the specific sequences. According to the specific sequences, 18 pairs of primers were designed with the Oli-go6. 4 program to amplify the specific fragments of dengue virus. The amplified fragments were cloned and sequenced. The method of preparing microarray probes by RT-PCR was effective, quick and simple.
出处
《生命科学研究》
CAS
CSCD
2004年第1期67-70,共4页
Life Science Research
