大鼠脑外伤后胎脑移植的增殖细胞核抗原和Bcl-2的表达

Expression of PCNA and Bcl-2 in the traumatic brains implanted with embryonic brain tissue in rats

目的 :研究大鼠脑外伤后胎脑移植的增殖细胞核抗原 (PCNA)和 Bcl- 2的表达。方法 :自由落体撞击法制作脑外伤模型 ,延迟 2周作创伤灶内胎脑细胞移植 ,伤后 6周观察创伤灶 HE染色 ,PCNA和 Bcl- 2的免疫组化染色、计数。结果 :伤后均表现为胶质细胞和毛细血管的增生 ,胎脑移植后更明显 ;PCNA表达两者基本相同 ;Bcl- 2在单纯损伤组未见表达 ,而在损伤后移植组明显表达。结论 :胎脑移植可促进创伤脑区 Bcl- 2表达 ,对脑外伤引起的神经细胞死亡具有保护作用。

Objective: To investigate the expression of PCNA and Bcl-2 in the traumatic brain area transplanted with embryonic brain tissue in rats. Methods: The cerebral contusion of rats was induced by dropping weight. The homogenates of embryonic brain tissue were transplanted into the traumatic brain area two weeks after injury. All rats were sacrificed 6 weeks after injury (4 weeks after transplantation), and their brains were examined histologically. The expressions of PCNA and Bcl-2 in the brains were analyzed by immunohistochemical methods. Results: The histology of brain presented the capillary and glia proliferation, especially in the transplantation group. No significant difference was found in the expression of PCNA between two groups. However, Bcl-2 was overexpressed in the transplantation group. Conclusion: The transplantation of the embryonic brain tissue enhances the expression of Bcl-2, which may play a neuroprotective role following traumatic brain injury.