不同碳源对三种溶磷真菌溶解磷矿粉能力的影响

Effect of different C sources on the solubilization of rock phosphate by three phosphate solubilizing fungi (PSF)

通过液体培养法 ,对 3种溶磷真菌利用葡萄糖、果糖、蔗糖、麦芽糖、淀粉和纤维素等碳源溶解宜昌产磷矿粉的试验 ,结果表明 ,菌株P2 3在供给葡萄糖时的溶磷能力最高 ,并在一定程度上能够利用长链碳源淀粉和纤维素为营养而溶磷 ;而高效溶磷菌株P6 6和P39溶磷的最佳碳源是果糖和麦芽糖 ,该两菌株利用淀粉和纤维素的溶磷效果很小 ,甚至不溶磷。 3种溶磷真菌培养滤液 pH值、可滴定酸含量与其溶磷量之间的相关性因菌株而异 ,差别很大。菌株P2 3培养滤液pH值、可滴定酸含量与其溶磷量之间相关性很低 ,但菌株P6 6和P39培养滤液pH值、可滴定酸含量与其溶磷量之间相关性却达到极显著水平 (P <0 0 1)。结果表明 ,不同碳源对溶磷菌溶解磷矿粉能力影响很大 ,分析推断 3种菌株产生的有机酸活化磷矿粉能力为P6 6>P39>P2 3。

Effect of different C sources (glucose, fructose, sucrose, maltose, starch and cellulose) on solubilization of rock phosphate by 3 phosphate solubilizing fungal strains was studied in liquid media. Strain P2.3 had the highest capacity to solubilize rock phosphate when supplied with glucose, and it also had the ability to use long chain C sources such as starch and cellulose to solubilize rock phosphate. However the strains P66 and P39 had the highest phosphate solubilization activities with fructose and maltose supply. But those strains used a little or no starch and cellulose to solubilize rock phosphate. The relationship among pH, titratable acidity values and soluble P concentration of the culture filtrate depended on the fungal strains. When inoculated with P2.3, the relationship among each other was very low. But when using P66 and P39, higher correlations appeared among each other. The experimental results showed that the ability differed in using different C sources to solubilize rock phosphate due to different kinds of organic acids produced by different phosphate solubilizing fungi.The rock phosphate solubilized by organic acids produced by different PSF was P66>P39>P2.3.