有机磷除草剂草甘磷(GPS)对作物细胞遗传学毒性效应的研究

Effects of Organo- Phosphine Herbicide Glyphosate on Cyto- Genetical Toxicity in Grasses

利用有机磷除草剂草甘磷(Glyphosate,GPS),在不同浓度、不同处理时间下,对作物(黑麦、大麦)根尖分生组织细胞有丝分裂的影响进行了研究。结果表明,在浓度为50mg·L-1、处理时间为4h时,能引起细胞异常有丝分裂如多极、染色体桥、间期微核形成、细胞有丝分裂指数降低。草甘磷对黑麦、大麦细胞异常有丝分裂的临界浓度和临界时间分别是50mg·L-1和4h。

Effects of organophosphine herbicide Glyphosate (GPS) on cyto- genetical toxicology were studied in root tips of Secale cereale and Hordeum vulgare treated with a series of concentrations of GPS and various durations. The results showed that the plant had abnormal cell mitosis, such as multipolar, chromosomes bridge and formation of micronuclei in interphase. Furthermore, the herbicide also caused reduction in mitotic index when root tips were treated with a dosage of 50 mg· L- 1 GPS for 4 h. We indicated from our test that the treatment concentration of 50 mg· L- 1 and exporsure time with 4h by the herbicide were believed to be the threshold concentration and threshold time for causing abnormal cell mitosis in Secale cereale and Hordeum vulgar, respectively.