hASB-8相互作用蛋白的初步研究

A Study of Interacting Proteins with hASB-8

hASB 8基因是对肿瘤细胞生长具有明显抑制作用的人类新基因 .其编码蛋白属于人ASB蛋白家族中的一个成员 ,与小鼠中的ASB 8蛋白同源性达 96 % .保守结构域分析显示hASB 8在N端包含 4个Ankyrinre peats ,在C端包含了一个SOCSbox .利用酵母双杂交技术 ,筛选了人的胎盘 (Placenta)cDNA文库 ,获得了与hASB 8相互作用的 2个蛋白 ,ElonginC和CDK4bindingprotein ;并在二倍体酵母体内进行了验证 .这些试验提示hASB 8蛋白可能介导肿瘤细胞中靶蛋白和泛素复合体之间的相互作用 。

hASB-8 is a human novel gene that has apparent effects on the growth of tumor cells. It is a new member of human ASB protein family, with a 96% homology to mouse ASB-8 protein. Conserved domain analysis indicated that it contained four Ankyrin repeats in its N terminal and one SOCS box in its C terminal. By using the yeast two-hybrid technology, a human placenta cDNA library was screened and 2 positive clones named Elongin C and CDK4 binding protein were obtained. Their interaction with hASB-8 was also tested in diploid yeasts. These results indicated that hASB-8 may mediate the interaction between target proteins and ubiquitination complex, and correlate with the transcription process of target genes in tumor cells.