摘要
本研究对吉林省内12个水域采集和送检的66尾病鱼进行细菌分离鉴定,共检出46株嗜水气单胞菌疑似株,通过生化试验和PCR扩增气溶素基因aer鉴定法,确定其中22株为嗜水气单胞菌。进一步采用纸片扩散法和双倍稀释法测定嗜水气单胞菌分离株对多种抗生素的耐药性,其中部分菌株存在不同程度的耐药性,22株嗜水气单胞菌中有4株对喹诺酮类药物耐药,并且为交叉耐药。其对喹诺酮类药物的抑菌圈均小于19mm。嗜水气单胞菌耐喹诺酮类药物的检出率为18 2%。将病料中分离的8株嗜水气单胞菌在电镜下观察发现,2株耐药菌表面发现均匀分布的球形结构,而6株敏感菌表面未见此结构。
Forty-six strains of Aeromonas. hydrophil isolates were isolated from the fishes collected from 12 water regions in Jilin province. Biochemical tests and amplified aer genes with PCR were utilized to identify the A. hydrophil isolates. Among forty-six isolates tested, 22 strains were identified as A. hydrophil and 4 drug resistant strains were found by drug-resistance routine agar diffusion tests and minimal inhibiting concentration tests. Norfloxacin, ciprofloxacin and ofloxacin resistance were verified. The identified strains, including 6 susceptible and 2 resistant strains, were cultured in common medium, washed with physiological saline, centrifugaled at indoor temperature, dyed with phosphorus wolfram acid, and observed on electric microscope. Many small globules with similar size were observed on the surface of the 2 drug-resistant strains instead of the surface of the 6 susceptible strains.
出处
《水产科学》
CAS
北大核心
2004年第4期20-23,共4页
Fisheries Science
基金
吉林省科学技术厅自然科学基金资助项目(20020648)
