类风湿关节炎患者血清sFas、sFasL和IL-18水平及意义

Serum levels of sFas, sFasL,IL-18 in patients with rheumatoid arthritis and its clinical significance

目的 探讨类风湿关节炎 (RA)患者血中可溶性Fas(sFas)、可溶性Fas配体 (sFasL)和白细胞介素 18(IL 18)水平及意义。方法 应用双抗体夹心酶联免疫吸附试验 (ELISA)和速率散射比浊法检测 70例RA患者血中sFas、sFasL、IL 18和类风湿因子 (RF)、C 反应蛋白 (CRP)含量。结果 RA患者sFas、sFasL、IL 18、RF、CRP含量分别为 ( 3.75± 1.79)ng/ml、( 12 15± 10 17)pg/ml、( 6 6 .6± 4 9.6 ) pg/ml、( 4 87±6 6 1)IU/ml、( 2 .75±4 .14 )mg/dl,与对照组 [( 2 .37± 1.2 9)ng/ml、( 4 43± 2 4 5 ) pg/ml、( 32 .2± 2 0 .5 ) pg/ml、<30IU/ml、<0 .0 1mg/dl]相比 ,差异有显著性 (P <0 .0 5 )。sFasL与CRP、RF呈正相关 (r =0 .5 36 ,r =0 .394 ,P <0 .0 5 ) ,sFas与IL 18呈正相关 (r =0 .80 8,P <0 .0 1)。结论 sFas、sFasL、IL

Objective To investigate the levels of soluble Fas(sFas), soluble Fas ligand(sFasL) and IL 18 in serum from patients with rheumatoid arthritis (RA) and its clinical significance. Methods Levels of sFas, sFasL and IL 18 in 70 patients with RA were measured by a double antibody sandwich ELISA. The serum levels of RF and CRP were also determined using Beckman Array 360. Results In patients, the levels of sFas, sFasL, IL 18, RF and CRP were as follows: (3.75±1.79) ng/ml, (1 215±1 017) pg/ml, (66.6±49.6) pg/ml, (487±661) IU/ml and ( 2.75 ±4.14) mg/dl, respectively. In healthy controls, they were as follows: ( 2.37 ±1.29) ng/ml, (443±245) pg/ml, (32.2±20.5) pg/ml, <30 IU/ml and <0.01 mg/dl, respectively. There was significant difference between RA and healthy control group( P <0.05). In patients, there were positive correlations between sFasL and CRP, RF( r =0.536, r =0.394, P <0.05), also between sFas and IL 18( r =0.808, P <0.01). Conclusions Serum levels of sFas, sFasL and IL 18 may be related with the course of RA.