幽门螺杆菌感染患者血清IL-8和TNFα的水平变化及临床意义探讨

Study on the levels of IL-8 and TNFα in serum of patients with Helicobacter pylori infection and its clinical significance

目的 监测幽门螺杆菌 (Helicobacterpylori,HP)感染患者血清白细胞介素 8(IL 8)和肿瘤坏死因子α(TNFα)的变化规律 ,探讨HP的致病机制。方法 用胃镜检查尿素酶试验、蛋白印迹法对HP进行定性分析、分组 ,酶联免疫吸附试验 (ELISA)检测IL 8、TNFα的表达水平。结果 高毒力型HP感染组患者血清IL 8、TNFα水平与低毒力型HP感染组、HP阴性组相比差异有显著性 (P <0 .0 1) ,HP感染者血液IL 8和TNFα呈正相关。结论 高毒力型HP的感染是IL 8和TNFα升高的主要因素 ,表明IL 8和TNFα在胃。

Objective To study the levels of IL 8 and TNFα in serum of patients with Helicobacter pylori (HP) infection and its clinical significance. Methods HP was determined and classified by the immunoblot assay and rapid urease test. The levels of IL 8 and TNFα were measured by ELISA. Results The diffierence of the levels of IL 8 and TNFα was significant between the high virulence HP group and the low or negative group( P <0.01). IL 8 correlated with TNFα in serum of patient with HP infection. Conclusions HP infection with high virulent factor is the main cause in the elevation of IL 8 and TNFα. IL 8 and TNFα may be important factors in inducing gastroduodenal disease.