mini VITAL全自动血培养仪假阳性/假阴性结果分析

Evaluation of false positive and false negative results for mini VITAL automated blood culture system

目的 评价miniVITAL全自动血培养仪在临床应用中的假阳性 /假阴性情况。方法 采用回顾性分析 ,对miniVITAL全自动血培养仪在检测 3834份临床标本中出现的假阳性 /假阴性率及导致假阴性结果的细菌种类与分布进行评估分析。结果 miniVITAL系统假阳性率和假阴性率分别为 0 .6 8%和 0 .70 % ,所有致假阴性菌种中 ,以真菌的比率最高 ,占 6 2 .0 7% ,其次为非发酵菌、草绿色链球菌、凝固酶阴性葡萄球菌、革兰阳性杆菌和厌氧菌 ,分别为 10 .34%、10 .34%、6 .90 %、6 .90 %和 3.4 5 %。

Objective To evaluation the false positive and false negative results for mini VITAL automated blood culture system in clinical application. Methods A total of 3 834 specimens were detected by the mini VITAL system. The false positive and false negative results were analyzed retrospectively. Results The false positive and false negative rates were 0.68% and 0.70%. Epiphyte was the main cause of the false negative results (62.07%), and the others were nofermenters (10.34%), Viridans streptococci (10.34%), coagulase negative Staphylococci (6.90%), Gram positive Corynebacterium (6.90%) and anaerobic bacteria (3.45%). Conclusions It is necessary to do subculture of bacterium and fungus for the negative results reported by mini VITAL system.