食管癌组织中肝素酶mRNA的表达

Relation between heparanase mRNA expression and metastasis of esopha-geal carcinoma

目的:探讨肝素酶mRNA表达与食管癌转移的关系。方法:采用原位杂交技术检测了54例食管鳞癌、癌旁组织及其正常食管组织中肝素酶mRNA的表达。结果:21例有淋巴结转移的食管鳞癌癌组织、癌旁及正常组织中肝素酶mRNA阳性表达率分别为100％(21/21)、66.7％(14/21)和0％(0/21)。而在无淋巴结转移的33例食管鳞癌癌组织、癌旁及正常食管组织中肝素酶mRNA阳性表达率分别为30.3％(10/33)、24.2％(8/33)和0%(0/33)。2组癌组织、癌旁组织肝素酶mRNA阳性表达率差异有统计学意义(P<0.01)。结论:肝素酶mRNA表达与食管鳞癌转移有一定关系。

Aim :To investigate the relation between the mRNA expression of heparanase and the metastasis of esophageal carcinoma. Methods: The expression of heparanase mRNA in 54 cases esophageal cancer including cancerous tissues, mucosa adjacent to cancer,and normal mucosa was detected by in situ hybridization. Results: The positive rates of heparanase mRNA in cancerous tissues, mucosa adjacent to cancer, and normal tissues of cases with lymph node metastasis were 100% (21/21) , 66. 7% ( 14/21 ) , and 0% , respectirely, and the positive rates of heparanase mRNA in cancerous tissues, mucosa adjacent to cancer, and normal tissues of cases without lymph node metastasis were 30. 3% (10/33) , 24. 2% (8/33) and 0% , respectirely. There was significant difference between esophageal carcinoma with lymph node metastasis and esophageal carcinoma without lymph node metastasis (P < 0. 01). Conclusion : The results suggest the expression of heparanase gene may be related to the metastasis of esophageal carcinoma.