神经母细胞瘤组织中Survivin的表达

Expression of Survivin in neuroblastoma

目的:探讨神经母细胞瘤组织中Survivin的表达。方法:运用原位杂交及TUNEL法检测26例神经母细胞瘤(NB)、9例神经节细胞瘤和10例正常肾上腺组织巾Survivin的表达以及细胞凋亡情况。结果:Survivin在NB组中的表达明显高于神经节细胞瘤组及对照组(P<0.01),其表达与患儿的年龄、临床分期及病理分型有关(P均<0.001);而凋亡指数(AI)则与NB的组织分型、临床分期及病理分型有关(P均<0.05)。在NB组织中Sur-vivin的表达与AI呈负相关(r=-0.696,P=0.000)。结论:Survivin可以作为一种新的NB诊断及预后判别的标志物,在调控NB的发生、发展、分化及凋亡方面非常关键。

Aim: To investigate the value of Survivin in neuroblastoma occurrence, development, clinical diagnosis and prognostic distinguishing. Methods: In-situ hybrization technique and TUNEL method were adopted for detection of expression of Survivin mRNA and apoptotic cell. Results: Expression of Survivin in neuroblastoma was obviously higher than those in ganglioneuroblastoma and normal adrenmedullary tissue (P<0.01). Expression of Survivin in neuroblastoma was related with age, clinical stage and histological classification (P <0.001 ) ,and expression of apoptosis was related with tissue type, clinical stage and histological classification(P <0.05). In neuroblastoma Survivin and AI manifested negative relation(r= -0. 696 ,P =0. 000). Conclusion: Survivin can be used as a novel diagnostic and prognostic distinguishing marker of neuroblastoma, and plays a pivotal role in regulating origination, growth, differentiation and apoptosis of neuro-blasloma.