SARS冠状病毒基因组进化与PCR检测引物的设计

The application of genomes alignment in the PCR identification of SARS coronavirus

目的:分析比较32株SARS冠状病毒基因组的同源性,研究SARS冠状病毒PCR检测引物对32株SARS冠状病毒的适用性。方法:运用互联网和生物信息学的方法、软件,分析32株SARS冠状病毒基因组之间的同源性,构建无根进化树,并对PCR检测引物进行了检索验证。结果:32株SARS冠状病毒的全基因组序列中共有488个位点的碱基发生突变,并且在所有32株SARS冠状病毒的基因组序列上,均存在PCR检测引物对应的序列片断。结论:所设计的SARS冠状病毒PCR检测引物在全部32株SARS冠状病毒的PCR检测分析中都是适用的。

Aim: To compare the homologous sequences of genes among 32 SARS coronaviruses genomes and prove the suitability of the primer of PCR identification for complete genomes of all the SARS coronaviruses. Methods: By using of the analysis data on internet and the softwares of bioinformatics, a phylogenetic tree was obtained and PCR primers were designed. Results: Four hundred and eighty eight substituted base sites were found in 32 SARS coronavirus complete genomes , and there was a same extended segment on the coronavirus genomes. Conclusion: The suitability of the designed primer of PCR identification for the coronavirus complete genomes has been proved.