卵母细胞单精子注射261个治疗周期分析

Analysis on 261 cycles of introcytoplasmic sperm injection

目的:阶段性回顾分析261个卵胞浆内单精子注射(ICSI)周期的实验室及临床结果。方法:收集1999年10月至2001年12月在本院生殖中心接受ICSI治疗的严重男性因素及不明原因不孕的患者261个治疗周期的资料,并分为两个阶段:1999年10月至2000年12月,2001年1月至2001年12月。分析比较两阶段的成熟卵母细胞率、受精率、卵裂率、Ⅰ级胚胎率、平均移植胚胎数、临床妊娠率、流产率和妊娠率与女方年龄的关系。结果:两阶段的成熟卵母细胞率、受精率、临床妊娠率分别为86.31％和91.00％,60.98％和73.50％,28.78％和38.52%,差异有统计学意义(P<0.05)。卵裂率、Ⅰ级胚胎率、平均移植胚胎数差异无统计学意义(P>0.05)。同时发现妇女年龄与临床妊娠率有关。结论:控制性超排卵获得高质量的卵子、女方年龄、ICSI的操作技术直接影响ICSI的胚胎质量,与妊娠率有密切关系。

Aim: To investigate the laboratory and clinic efficacy of 261 ICSI cycles retrospectively. Methods: From October 1999 to December 2001 ,261 cycles received ICSI in our assisted reproductive center due to severe male factor infertility or idiopathic infertility. The treated cycles were divided into two groups. Group I was from October 1999 to December 2000, Group Ⅱ from January 2001 to December 2001. Result: Mature oocyte rate(86. 31% vs 91.00% ) , fertilization rate (60.98% vs 73. 50% ) and clinical pregnancy rate (28. 78% vs 38. 52% ) , were significantly higher in Group Ⅱ than those in Group Ⅰ(P<0.05). No differences were observed between two groups in cleavage rate, Grade Ⅰ embryo rate, and mean number of transferred embryos ( P > 0.05). Statistics analysis were not done on multi-pregnancy rate and abortion rate due to fewer cases. The study data showed that the abortion rate and multi-pregnancy rate gradually went down with the improvement of fertilization rate and the decreasing of mean number of transferred embryos. Clinical pregnancy rate was connected with the female patient age. Conclusion: The high quality oocyte#the female patient age and the ICSI technique do affect the quality of embryo and pregnancy rate.