青石棉诱导A549细胞ERK1/2、MEK1/2的表达

Activation of mitogen-activated protein kinase signaling pathway in human airway epithelial cells exposed to crocidolite

目的:了解细胞内信号传导在石棉致肺部疾患过程中的作用。方法:用Western免疫印迹法检测石棉刺激的A549细胞裂解液中MEK1/2、:ERK1/2的表达。结果:以0.1 g/L浓度的青石棉刺激A549细胞后,ERK1/2、MEK1/2磷酸化蛋白快速高表达,与空白对照比较,差异有统计学意义(P<0.05)。结论:磷酸化的信号蛋白可能在石棉致病中起重要作用。

Aim :To study the role of intracellular signaling transduction pathway in the process of the pulmonary diseases induced by crocidolite fibers. Method: Western blotting was employed to detect the expression of phosphorylated ERK1/2 and MEK1/2 in human bronchial airway epithelial cell A549 stimulated by crocidolite. Results: Using the concentration of 0. 1 g/L crocidolite fiber, it showed that the expression of phosphorylated ERK1/2 and phosphorylated MEK1/ 2 were striking higher than those of the blank control, the differences were significant (P <0.05). Conclusion: Activated ERK1/2 and MEK1/2 probably involved in the process of the pulmonary diseases induced by crocidolite.