编码流感病毒血凝素基因和CD40L的核酸疫苗抗小鼠流感研究

Plasmid Encoding CD40 Ligand Enhances Immune Response to Influenza DNA Vaccine

为了检测表达CD40L的质粒能否作为核酸疫苗佐剂提高A/PR8/34流感病毒血凝素(HA)DNA疫苗的免疫应答,构建了表达鼠CD40L的质粒,并将它与A型流感病毒的 HA DNA疫苗用电击的方法共同免疫BALB/C小鼠(各30礸),免疫两次,间隔三周,第二次免疫后1周,用致死量流感病毒A/PR8/34攻击。发现与单独免疫30礸 HA相比,血清中抗HA的IgG抗体量明显提高,且以IgG2a抗体提高为主,小鼠体重减轻非常少且体重恢复加快。实验结果显示,CD40L能作为流感病毒核酸疫苗佐剂,提高小鼠抗流感病毒攻击的能力。

CD40L gene can act as a genetic adjuvant. The objective of this study was to test the effectiveness of CD40L-expressing plasmid on the immune response to influenza A/PR/8 virus hemagglutinin (HA) DNA vaccine. To achieve this, we constructed plasmid DNA encoding murine CD40L and coimmunnized the plasmid with HA DNA to the BALB/c mice muscle by electroporation. Each DNA at a dose of 30 靏 was administered twice at a 3-week interval. One week after the second vaccination, the mice were challenged with a lethal dose of influenza A/PR/8 virus. We found that, after lethal influenza virus challenge, CD40L and HA DNA-coimmunized mice exhibited higher induction of specific IgG (in particular IgG2a) against HA as well as lower body weight loss than those of HA DNA immunized alone. We concluded that CD40L gene had adjuvant effects on influenza DNA vaccine.