绵羊肺腺瘤病毒中国NM株部分gag基因的克隆与序列分析

Partial Cloning and Sequence Analysis of the gag Gene of Jaagsiekte Sheep Retrovirus NM Strain

参照GenBank中已发表的绵羊肺腺瘤病毒的基因序列,设计合成一对引物,对绵羊肺腺瘤病毒(Jaagsiekte sheep retrovirus,JSRV)内蒙株的gag基因中主要编码CA蛋白的基因段进行PCR扩增,产物经琼脂糖凝胶电泳分析,呈现一条约897bp的特异条带,将其回收后克隆入pMD-18 T载体中,并进行序列测定与分析。结果表明,与南非株(基因序列号NC-001494)的gag基因序列比较,核苷酸同源性为83%,推导出的氨基酸同源性为84%。与美国株(基因序列号AF105220)的gag基因序列比较,核苷酸同源性为81.5%,氨基酸同源性为83%。这是我国首次报道的绵羊肺腺瘤病毒的gag基因的一段序列,为我国科研工作者进行更深入的研究奠定基础。

In order to amplify partial gag gene of Jaagsiekte sheep retrovirus Inner Mongolia strain, a pair of primers was designed according to the GenBank sequence . The fragment of gag gene was obtained by polymerase chain reaction (PCR), and the gene was cloned into PMD-18 T vector and identified by EcoR I and Sal I digestion. The nucleotide and amino acid sequences of NM strain partial gag gene were compared with the counterpart sequeuces of South Africa strain (Accession No. NC-001494) and USA strain (Accession No. AF105220). The nucleotide and amino acid homology of partial gag gene were 83%, 81.5% and 84%, 83%, respectively. This is the first JSRV nucleotide sequence reported in China.