RBSDV在玉米叶脉细胞内的侵染状态与灰飞虱传毒活力的关系

Study on the Relation Between RBDV Infecting Stages in Maize Vein Cell and Active Transmission of Laodelphax striatellus Fallen

根据水稻黑条矮缩病毒(RBSDV)侵染玉米(Zea mays L.)的症状发展过程先后取叶脉做超薄切片,在透射电镜下观察病毒在细胞内的侵染状态,并在取样前用灰飞虱无毒若虫进行饲毒和传毒试验。结果显示RBSDV侵入玉米叶细胞后先出现在细胞壁附近,个别粒子似与胞间连丝相连;细胞质内产生病毒基质,病毒粒子先增殖并分布其周边,后向病毒基质内扩展;当病毒粒子布满病毒基质后在细胞质中出现直径约90nm的管状结构,病毒成串排列在该管状结构中;随后管状结构逐渐消失,最终形成晶格状聚集排列。用灰飞虱无毒若虫在细胞内病毒基质出现和病毒增殖期饲毒的,到成虫时分别有2.93%和7.83%个体传毒率;在细胞内病毒成串分布于管状结构和晶格状聚集排列期饲毒的,到成虫时均不能传毒。

Rice black streaked dwarf virus (RBSDV) infecting stages were viewed in maize vein cell during its different infection period by electron microscope. It was found that the RBSDV particles infected in the maize cell appeared at first in the cytoplasm near the cell wall, some single virus particles seem to be connected with plasmodesma. The viroplasm was produced in cytoplasm, and the RBSDV particles multiplied and scattered initially along the periphery and subsequently spread out towards the viroplasm. When the viroplasm was full of virus particles, some tubules with about 90nm in diameter appeared in the cytoplasm. Subsequenctly the virus particles arrayed in row among them, and finally the particles aggregated into crystalline array and the cytoplasm tubules disappeared. The percentage of active transmission of small brown plant hopper (Laodelphax striatellus Fallen) varied with the infection stage of RBSDV in maize cell while the nymph acquiring feeding. There were 2.93% and 7.83% of viruliferous rate respectively when their nymph acquiring feeding in the vein cell of maize among RBSDV particles were going to multiply in the periphery and inner of viroplasma, but no one when the noninfective nymph of them acquiring feeding in maize cell among the virus particles was going to the stage of appearing cytoplasm tubules and forming crystalline array.