从贵州白纹伊蚊体内分离到一株2型登革病毒

Isolation Identification and Phylogenetic Analysis of A New Dengue Virus Strain from Field Aedes albopictus in Mawei Town

从贵州省独山县麻尾镇白纹伊蚊标本中分离到一株病毒 ,用抗DEN 1 4型单克隆抗体经间接免疫荧光法和RT PCR产物酶切分型鉴定为DEN 2 ,并对其NS1和E基因RT PCR产物进行部分基因序列测定 ,与DEN 2NGC株比较 ,麻尾株的NS1基因区有 7个碱基发生点突变 ,E基因区有 1个碱基的插入 ,两个序列被GenBank收录 ,编号分别为AY2 774 0 2、AY2 782 2 6 ;麻尾分离株与其他 9株DEN 2型病毒进行系统发育关系的分析 ,结果显示与D2 4 3株系统进化关系最近 ;证明贵州省存在DEN感染的自然循环。

One new strain of dengue virus was isolated from field Aedes albopictus in Mawei town of Guizhou Province. It was identified as dengue virus 2 type by IFA with monoclonal antibody against dengue virus 1-4 types and RT-PCR. The E and NS1 share gene of the new strain were sequenced. Seven point mutations of NS1 gene and one base insertion of E gene were found, comparing with DEN-2 NGC strain. This different sequence were accepted by GenBank. The accession number of NS1 、E gene were AY277402 、 AY278226, respectively. A phylogenetic tree was constructed by comparing with the published E 、NS1 gene sequence from nine strains of dengue virus 2 type.These findings highlighted the existence of a silent epidemic in Guizhou. Dengue virus 2 type Mawei strain is more related to D2-43 than others.