期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

阿维链霉菌bkdF的基因中断对阿维菌素合成的影响 被引量：3

Effects of bkdF Interruption on Avermectin Biosynthesis
下载PDF
导出
摘要 以阿维菌素B组分菌株StreptomycesavermitilisBjbm0 0 0 6为出发菌株 ,用PCR方法构建支链α 酮酸脱氢酶基因bkdF(Branched chainα ketoaciddehydrogenasegene)的重组质粒pHJ5 816 (pHZ135 8 bkdF&Ermr)对其进行基因中断 ,得到重组菌株Bjbm5 816。经HPLC检测和核磁共振分析发现 ,Bjbm5 816发酵产物产生的单一组分新化合物为Oligo mycinA。 Bjbm0006 was a strain which produces four avermectin B component. bkdF (branched-chain α-keto acid dehydrogenase gene) gene involved in the biosynthesis of avermectin B was cloned by PCR from Bjbm0006 chromosome, and inserted with a Erm r gene to construct a recombinant plasmids pHJ5816 (pHZ1358∷bkdF&Erm r). The bkdF-interrupted mutant Bjbm5816 was received through homologous double crossover between the pHJ5816 and the chromosome. The interruptant Bjbm5816 produce single novel compound and it was attested to be Oligomycin A by MS, 1H-NMR, 13 C-NMR and two-dimension NMR.
作者 朱浩君 梁运祥 周俊初 郑应华
机构地区 华中农业大学农业微生物重点实验室 北京生物医药研究所
出处 《微生物学报》 CAS CSCD 北大核心 2004年第2期194-197,共4页 Acta Microbiologica Sinica
关键词 阿维链霉菌 阿维菌素 基因中断 支链a-酮酸脱氢酶基因 菌株 生物合成 Avermectin, Branched-chain α-keto acid dehydrogenase complex, Gene interruption
分类号 Q78 [生物学—分子生物学]
  • 相关文献

参考文献14

  • 1Skinner D, Morgenstem M, Fedechko R, et al. Cloning and sequencing of a cluster of genes encoding branched-chain α-keto acid dehydrogenase from Strcptomyces averrnitilis and the production of a functional E1 [αβ] component in Escherichia coli. J Bacteriol,1995, 177, 183-190.
  • 2Denoya C, Fedechko R, Hafner E, et al. A second branched-chain α-keto acid dehydrogenase gene cluster (bkdFGH) from Streptomyces avermitilis : its relationship to avermectin biosynthesis and the construetion of a bkdF mutant suitable for the production of novel antiparasitic avermectins. J Bacteriol, 1995, 177, 3504 - 3511.
  • 3Zhu H J, Zheng Y H. Study on C5-O-methyltransferase gene cloning, sequencing and replacement. J Chin J Antibiotics, 2003, 28 ( 1 ) : 1-5.
  • 4Sambrook J, Fritsch E F, Maniatis T. Molecular Cloning : A laboratory Manual. 2^nd ed. New York: Cold Spring Harbor Laboratory Press, 1989.
  • 5Tobias K, Bibb M J, Mark J B, et al. Practical Streptomyces Genetic. Norwich: The John Innes Foundation, 2000.
  • 6MacNeil T, Gewain K, MacNeil D. Deletion analysis of the avermectin biosynthetic genes of Streptomyces avermitilis by gene cluster displacement. J Bacteriol, 1993, 175:2552 - 2563.
  • 7Ikeda H, Nonomiya T, Omura S. Organization of biosynthetic gene cluster for avermectin in Streptomyces avermitilis : analysis of enzymatic domains in four polyketide synthases. Journal of Industrial Microbiology & Biotechnology, 2001, 27, 170 - 176.
  • 8Skinner D, Morgenstern M, Fedechko R, et al. Cloning and sequencing of a cluster of genes encoding branched-chain α-keto acid dehydrogenase from Streptomyces avermitilis and the production of a functional E1[αβ] component in Escherichia coli. J Bacteri
  • 9Denoya C, Fedechko R, Hafner E, et al. A second branched-chain α-keto acid dehydrogenase gene cluster (bkdFGH) from Streptomyces avermitilis : its relationship to avermectin biosynthesis and the construction of a bkdF mutant suitable for the production o
  • 10Zhu H J, Zheng Y H. Study on C5-O-methyltransferase gene cloning, sequencing and replacement. \%J Chin J Antibiotics,\% 2003, 28 (1):1-5.

同被引文献59

  • 1范铭琦,赵敏.氨基糖苷类抗生素产生菌的代谢调控与菌种选育[J].中国抗生素杂志,2005,30(9):576-580. 被引量：6
  • 2Burg R W,Miller B M,Baker E E,et al.Avermectins,new family of potent anthelmintic agents:producing organism and fermentation[J].Antimicrob Agents Chemother,1979,15 (3):361～367.
  • 3Omura S,Ikeda H,Ishikawa J,et al.Genome sequence of an industrial microorganism Streptomyces avermitilis:deducing the ability of producing secondary metabolites[J].Proc Natl Acad Sci U S A,2001,98(21):12215～12220.
  • 4MacNeil D J.Characterization of a unique methyl-specific restriction system in Streptomyces avermitilis[J].J Bacteriol,1988,170(12):5607～5612.
  • 5Kieser T,Bibb M J,Buttner M,et al.Practical Streptomyces Genetics[M].Norwich:The John Innes Foundation,2000.
  • 6Cropp T A,Wilson D J,Reynolds K A.Identification of a cyclohexylcarbonyl CoA biosynthetic gene cluster and application in the production of doramectin[J].Nat Biotechnol,2000,18 (9):980～983.
  • 7Dutton C J,Gibson S P,Goudie A C,et al.Novel avermectius produced by mutational biosynthesis[J].J Antibiot (Tokyo),1991,44(3):357～365.
  • 8Sambrook J,Fritsch E F.,Maniatis T.Molecular Cloning:A Laboratory Manual[M].New York:Cold Spring Harbor Laboratory Press,1989.
  • 9Flett F,Mersinias V,Smith C P.High efficiency intergeneric conjugal transfer of plasmid DNA from Escherichia coli to methyl DNA-restricting streptomyeetes[J].FEMS Microbiol Lett,1997,155(2):223～229.
  • 10Redenbach M,Kieser H M,Denapaite D,et al.A set of ordered cosmids and a detailed genetic and physical map for the 8 Mb Streptomyces coelicolor A3 (2) chromosome[J].Mol Microbiol,1996,21 (1):77～96.

引证文献3

二级引证文献12

微生物学报
2004年 第2期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部