石油生物催化脱硫菌Agrobacterium tumefaciens UP3的分离筛选

Isolation of Dibenzothiophene-degrading Bacterium Agrobacterium tumefa UP3

从胜利油田被原油污染的土壤中筛选到一株能有效降解模型化合物二苯并噻吩 (DBT)的菌株。根据常规的形态分析、生理生化性状及 16SrDNA序列分析 ,将其鉴定为根癌土壤杆菌 (AgrobacteriumtumefaciensUP3)。该菌不能以十二烷、十六烷、液体石蜡和萘作为唯一碳源和能源生长 ,具有工业应用的潜力。对该菌株DBT降解能力的初步研究表明 ,5 4h内可将 5 0 0mg L的DBT降解至 15 0mg L。对降解产物的分析表明 ,根癌土壤杆菌降解DBT的途径与Kodama路线及 4_S路线不同。

A dibenzothiophene(DBT)_degrading bacterium was isolated from oil_contaminated soil from Shengli oil field by enrichment technique. Traditional taxonomy, assays of its 16S rDNA sequence homology identified it as Agrobacterium tumefaciens UP3. This strain cannot grow on n_dodecane, n_hexadecane, liquid olefin and naphthalene as its sole carbon and energy source, so this strain can be used in industry potentially. The DBT degrading ability of this bacterium was studied. The content of DBT decreased from 500 mg/L to 150 mg/L within 54 hours. The analysis of the metabolic product of DBT shows that the degrading mechanism of Agrobacterium tumefaciens is different from Kodama and 4_S mechanism.