新砷化合物对小鼠C26移植性肿瘤细胞体内生长抑制作用的研究

Inhibitory effect of new arsenide (AsI_3-3Tu) on the growth of the transplanted sarcoma C26 in vitro

目的 探讨新砷化合物 (AsI3 - 3Tu)对C2 6移植性肿瘤细胞的抑制作用及其对肿瘤组织环氧合酶 - 2(COX - 2 )及微血管密度 (MVD)的影响。 方法 将Balb/c小白鼠随机分为对照组、5 Fu组和AsI3 - 3Tu组 ,于接种C2 6移植性肿瘤细胞后第 6天开始腹腔注射给药 ,隔天 1次 ,共给药 8次 ,观察抑瘤率 ,并采用免疫组织化学的方法研究AsI3 - 3Tu对肿瘤组织COX - 2及MVD的影响。 结果 AsI3 - 3Tu对C2 6移植性肿瘤有明显的抑制作用 ,抑瘤率为 5 5 .0 7%。免疫组化结果显示 ,AsI3 - 3Tu对肿瘤组织的COX - 2表达有明显的抑制作用 ,同时MVD明显降低。 结论 AsI3 - 3Tu对C2 6移植性肿瘤有抑制作用 ,其作用途径可能是通过抑制COX - 2的表达而抑制肿瘤血管的形成 。

Objective To probe the effects of new arsenide (AsI 3-3Tu) on transplanted sarcoma C26 and on the expression of COX-2 and MVD in tumor tissue. Methods Balb/c mice were injected with transplanted sarcoma C26 and were randomizedly divided into control group,5-Fu group and AsI 3-3Tu group.The inhibitory rate on C26 was caculated routinely.The expression of COX-2 and MVD was detected by immunohistochemistry. Results The growth of C26 was significantly inhibited by AsI 3-3Tu,with the inhibitory rate of 55.07%(P<0.05).The expression of COX-2 in the tumor tissue was also inhibited by AsI 3-3Tu.And accordingly the expression of MVD was also markedly inhibited by AsI 3-3Tu. Conclusion AsI 3-3Tu had inhibitory effects on C26,and it decreased the expression of COX-2 in tumor tissue.There was positive relation between the expression of COX-2 and angiogenesis related factor.