肠型胃癌基因表达的cDNA微阵列分析

Gene expression profiling in intestinal-type gastric carcinoma by cDNA microarray

目的:研究肠型胃癌发生发展的基因表达变化. 方法:利用肠型胃癌和相应非癌组织的mRNA通过逆转录方法,将Cy3和Cy5两种荧光分别标记到两种cDNA上制成探针,然后与表达谱芯片(含4 096条基因)进行杂交后扫描,通过计算机辅助判别基因的差异表达. 结果:肠型胃癌和相应非癌组织组织中差异表达的基因有666条,上调表达333条,下调表达333条.参与细胞增生、凋亡、分化和转移调控的多种基因的表达水平发生了明显改变. 结论:高通量、高灵敏度的cDNA微阵列芯片技术提供了全面了解肠型胃癌基因表达谱的新思路,一些与肿瘤发生相关的差异表达基因有可能发展成为生物标记物或肿瘤早期诊断和治疗的靶点.

AIM: To identify a set of genes involved in the development of intestinal-type gastric carcinoma. METHODS: Pure mRNAs from 6 cases of intestinal-type gastric carcinoma and corresponding noncancerous mu-cosae were reversely transcribed into cDNAs labeled with Cy5 and Cy3 dyes for probes, then mixed and hybridized with the cDNA microarray consisting of 4 096 genes, and the fluorescent signals were scanned. RESULTS: Among total genes, 333 were up-regulated and 333 down-regulated in intestinal-type gastric cancer tissues. Within altered expression of those genes, cell-cycle regulators and growth factors were up-regulated, and the promoter genes of apoptosis were down-regulated; Oncogenes and cell-adhesion molecules were more up-regulated; The cancer progression genes were up-regulated, while the anti-cancer progression genes were down-regulated. CONCLUSION: The quick and high-throughout method of gene expression profile by cDNA array provides us with an overview of gene changes that may involved in intestinal-type gastric cancer development, and will open up new possibilities to identify novel molecular targets for diagnosis and therapy. Several genes are altered in intestinal-type gastric cancer, which need to be further investigated.