应用基因表达谱芯片技术克隆甘草甜素诱导Jurkat细胞后的差异表达基因

Cloning of genes differentially expressed in Jurkat cells treated with glycyrrhizin using cDNA microarray

目的:应用基因芯片技术,阐明甘草甜素作用于T淋巴细胞之后,甘草甜素对于T淋巴细胞基因表达谱的影响. 方法:应用基因表达谱芯片技术,对甘草甜素诱导的Jurkat 细胞和以生理盐水处理的相同细胞的mRNA进行差异显示分析,研究甘草甜素诱导人T淋巴细胞系Jurkat细胞后的差异表达基因. 结果:Jurkat细胞经甘草甜素诱导后,所检测的1152条目的基因中有30条产生差异表达,其中12条基因表达增强, 18条基因表达降低.表达增强的基因主要有:胸腺素及促胸腺生成素蛋白编码基因;白介素-18(IL-18);细胞代谢相关酶类.表达降低的基因主要有细胞信号转导相关基因(如血清/糖皮质激素调节激酶、丝裂素活化的蛋白激酶激酶激酶2、磷脂酶2调节亚基β、鸟嘌呤核苷结合蛋白、神经营养的酪氨酸激酶3型受体等);La自身抗原. 结论:应用基因表达谱芯片成功筛选了甘草甜素诱导T淋巴细胞后差异表达基因,为进一步阐明甘草甜素的免疫调节机制及深入了解甘草甜素用于防治病毒性肝炎的药理作用机制提供依据.

AIM: To study the difference in gene expression profile in human lymphoma cell line Jurkat cells treated with glycyr rhizin (GL), and to further elucidate the molecular immune mechanism of glycyrrhizin against T lymphocyte. METHODS: cDNA microarray technology was employed to detect the mRNA from Jurkat cells treated with GL and 0.9 percent sodium chloride, respectively. RESULTS: The results indicated that among 1 152 genes which were obtained from gene expression profile analysis, there were 30 genes different from those in GenBank in which 12 genes were up-regulated and 18 genes were down-regulated in Jurkat cells treated with GL, compared to those treated with 0.9 percent sodium chloride. These genes differentially regulated by GL included human genes encoding proteins involved in immune regulation, cell signal transduction, cell proliferation and differentiation. CONCLUSION: cDNA microarray technology is successfully used to screen the genes differentially expressed in Jurkat cells treated with GL, which brings some new clues for studying the immune regulation mechanism of GL.