幽门螺杆菌对肝细胞系HepG2 cyclinD1,PCNA mRNA表达的影响

Influence of H pylori on cyclinD1 and PCNA mRNA expression in HepG2 cell line

目的:研究幽门螺杆菌(H pylori)处理对肝细胞系HepG2 cyclinD1,PCNA mRNA表达的影响. 方法:将H pylori与HepG2共同培养1 h、3 h、6 h、12 h 和24 h,采用半定量RT-PCR方法分析共培养不同时间后HepG2 cyclinD1,PCNA mRNA的表达水平. 结果:CagA+ H pylori与HepG2细胞共同孵育1 h后,即可见cyclinD1 mRNA表达升高,3h达高峰,约为对照的4.0倍(P<0.01);共同孵育3 h后即可见PCNA mRNA表达升高,6 h达高峰,约为对照的2.0倍(P<0.05).而CagA- H pylori 和HepG2细胞共同孵育后,未见两基因的表达升高. 结论:CagA+ H pylori能诱导HepG2 cyclinD1,PCNA mRNA表达升高,提示其在肝癌的发生中起一定作用.

AIM: To observe the effects of H pylori on cyclinD1 and PCNA mRNA expression in a human hepatoma cell line HepG2. METHODS: H pylori was co-cultured with HepG2 for 1, 3, 6,12 and 24 hours. The cyclinDl and PCNA mRNA expression was detected by semi-quantitative RT-PCR. RESULTS: When HepG2 cells were cocultured with H pylori CagA+ strain, the amount of cyclinDl mRNA was increased 4.0-fold by 3 hours and PCNA mRNA was increased 2.0-fold by 6 hours, compared with that of uninfected control. Neither cyclinD1 mRNA nor PCNA mRNA of the HepG2 cells was increased after incubation with H pylori CagA- strain. CONCLUSION: H pylori can induce increasing expression of cyclinD1 and PCNA mRNA in HepG2, which may play some roles in the development of hepatocellular carcinoma.