COX-2与MMP-2蛋白在胃癌组织中的表达及意义

The significance of COX-2 and MMP-2 protein expression in human gastric carcinoma

目的 探讨COX 2和MMP 2蛋白在胃癌组织中的表达、相互关系及意义。方法 应用免疫组化S P法检测 45例胃癌及 2 0例正常胃组织MMP 2、COX 2蛋白的表达情况。结果 45例胃癌组织COX 2的表达阳性率为 60 % ( 2 7/ 45 ) ,2 0例正常胃组织COX 2的表达阳性率为 15 %O( 3 / 2 0 ) ,COX 2在胃癌组织中的表达阳性率显著高于正常胃组织中的表达阳性率 (P <0 0 1) ;45例胃癌组织中MMP 2的表达阳性率为 64 4% ( 2 9/ 45 ) ,2 0例正常胃组织MMP 2表达阳性率为 2 5 % ( 5 / 2 0 ) ,MMP 2在胃癌组织中的表达阳性率显著高于在正常胃组织中的表达阳性率 (P <0 0 1) ;在胃癌组织中MMP 2、COX 2蛋白的表达之间存在显著正相关 (rs=0 5 69,P <0 0 1)。结论 胃癌组织中存在COX 2、MMP 2的高表达 ,且两者之间的表达强度具有等级相关性。COX 2蛋白可通过诱导MMP 2蛋白的表达上调 ,增加胃癌细胞的侵袭力 ,从而成为其促进胃癌浸润。

Objective To study the significance of COX-2,MMP-2 protein expression and thrie relationships in human gastric carcinoma.Methods COX-2 protein and MMP-2 protein expression were examined by immunohistochemical S-P staining method in 45 cases of human gastric cancer and 20 cases of human normal gastric tissue using COX-2 polyclonal antibody and MMP-2 monoclonal antibody.Results Increased expression of COX-2 protein was present in the cytoplasm of cancer cell in gastric carcinoma;27 out of the 45 cases were COX-2 positive (60%),which was significantly hogher than that in normal gastric mucosa (P<0.01);Increased MMP-2 expression of protein was present in the cytoplasm and cytomembrane of cancer cell in gastric carcinoma;29 out of the 45 cases were MMP-2 positive (64.4%),which was significantly higher than that in normal gastric mucosa (P<0.01);The expression of MMP-2 was significantly correlated with COX-2 (r s=0.569,P<0.01).Conclusions The overexpression of COX-2 and MMP-2 protein were found in gastric carcinoma;To stimulate MMPs especially MMP-2 overexpression may be one of the pathways of COX-2 protein to contribute to the invasion and metastasis in human gastric carcinoma.