大鼠牙囊细胞集落刺激因子1受体的研究

Colony-stimulating factor-1 receptor in rat dental follicle cells

目的 研究不同浓度集落刺激因子 1(colony stimulatingfactor 1,CSF 1)、白细胞介素 1α(interleukin 1α ,IL 1α)对大鼠牙囊细胞CSF 1受体基因表达的影响 ;明确CSF 1自分泌作用的抑制机制 ,为研究细胞因子在牙齿萌出和牙槽骨吸收中的作用奠定基础。方法 间接免疫酶标法检测体外培养牙囊细胞 ,以及出生后不同时期牙囊组织中CSF 1受体蛋白表达。逆转录多聚酶链反应法 (RT PCR)检测在细胞培养液中分别加入不同浓度CSF 1、IL 1α对牙囊细胞CSF 1受体基因表达的影响。结果 间接免疫酶标法染色显示CSF 1受体蛋白在生后早期牙囊中染色明显 ,10d后染色不明显或者完全无染色 ,高浓度CSF 1作用下 ,牙囊细胞CSF 1受体mRNA表达明显消失。不同浓度IL 1α作用后 ,CSF 1受体mRNA水平保持不变。结论 CSF 1受体蛋白在出生后早期牙囊中表达明显 ,随后逐渐消失。高浓度CSF 1抑制CSF 1受体基因的表达 ,不同浓度IL 1α对牙囊细胞CSF 1受体mRNA表达无影响。

Objective To study localization and expression of CSF-1 receptor protein, in order to discover the CSF-1 and IL-1α effects on CSF-1 receptor mRNA levels and to determine if the autocrine effect is inhibited through the CSF-1 receptor. Methods Immunolocalization of CSF-1 receptor in the cultured dental follicle cells and in mandibles of the post-natal rats from day 1 to 11 were performed. The effects of different concentrations of CSF-1、IL-1α on CSF-1 receptor gene expression were detected by means of RT-PCR. Results Cultured dental follicle cells were immunostained for the CSF-1 receptor. In vivo, immunostaining showed that the CSF-1 receptor was present in the dental follicle of the first mandibular molar at early post-natally and was either absent or greatly reduced by day 11 post-natally. High concentrations of CSF-1 reduced the gene expression of the CSF-1 receptor. IL-1α had no effects on CSF-1 receptor mRNA levels.Conclusions The expression of CSF-1 receptor reaches a peak early post-natally in the dental follicle of the first mandibular molar of the rat and then subsequently declines. High concentrations of CSF-1 inhibites the expression of CSF-1 receptor, IL-1α has no effect on the expression of CSF-1 receptor mRNA.