L-硝基精氨酸对大鼠创伤性面瘫恢复的影响

Effects of L-nitroarginine on the recovery of traumatic facial paralysis

目的 研究组成型一氧化氮合酶 (constitutivenitricoxidesynthase ,cNOS)抑制剂L 硝基精氨酸 (L NGnitroarginine ,L NNA)对大鼠创伤性面瘫恢复的影响及面神经核内cNOS、小胶质细胞标志物(OX4 2 )表达的变化。方法 大鼠腹膜内给予L NNA ,在伤后各时间点观察面瘫的恢复 ,并观察面神经核内cNOS、OX4 2阳性神经元的变化。 结果 L NNA给药可显著延缓创伤性面瘫的恢复 ,其面神经核内cNOS免疫反应明显受到抑制 ;而OX4 2免疫反应明显提高。结论 内源性一氧化氮 (nitricoxide,NO)介质在创伤性面瘫模型中具有重要的介质作用。

Objective To study the effects of constitutive nitric oxide synthase inhibitor L-nitroarginine on the recovery of traumatic facial paralysis in rats and the changes of the expression of cNOS and OX42 in the facial nucleus. Methods L-nitroarginine was intraperitoneally injected into rats and the recovery of facial paralysis was observed at different time point. and the changes of cNOS and OX42 positive neurons were studied in facial nucleus. Results Treatment of L-nitroarginine could remarkably inhibit the recovery of traumatic facial paralysis. The cNOS immunoactivity was obvious inhibited in facial nucleus, while the OX42 immunoactivity was obvious increased. Conclusion Endogenous nitric oxide may play an important mediator role on the recovery of traumatic facial paralysis.