断层皮片成纤维细胞原代培养法

The fibroblast primary cell culture by the split-thickness skin slide technique

目的 在人成纤维细胞的生物学特性观察研究中 ,进行成纤维细胞的原代培养 ,以获得足够量的成纤维细胞。方法 我们采用整形外科断层取皮技术 ,使用细胞刮刀 ,可相对简单获取有活力的成纤维细胞 ,并应用台盼蓝细胞活力染色排除法进行检测。结果 该方法简单、经济可获取足够量的成纤维细胞 ,使得短期内即可获得大量的传代细胞。 1cm× 2cm的断层皮片可达到的细胞的数量级 10 6 左右。结论 断层皮片成纤维细胞培养方法 ,较胰酶消化法和组织块贴壁法有明显的优越性 ,在进行成纤维细胞移植方面 ,值得加以推广和应用。

Objective To acquire lots of cell to culture during the primary cell culture. Method We take the split|thickness skin slide technique to acquire the dissociated fibroblast cell in two big|ear rats. Results The cell number is above 10+6 from 1 cm×2 cm split|thickness skin slide and the technique is simple, economic,effectve. Conclusion We think this way is better than other methods,and should be adopted in the primary cell culture, especially in fibroblast transplantation by injection.