转基因星形胶质细胞脑内移植治疗帕金森病大鼠的研究

Intracerebral transplantation of genetically modified astrocytes for treatment of Parkinsonian rats.

目的 评价酪氨酸羟化酶 (TH)基因转染的星形胶质细胞移植入帕金森病 (PD)模型大鼠脑内后对旋转行为改善作用。方法 用pcDNA3 1/TH质粒转染原代培养的星形胶质细胞 ,采用免疫组化及RT PCR方法检测到TH表达后 ,将转基因的星形胶质细胞移植入PD模型大鼠脑纹状体内 ,观察PD大鼠的旋转行为变化情况。结果 移植后共观察 12周 ,转基因细胞移植组PD大鼠 (n =10 )的旋转行为明显改善 (P <0 0 5 ) ,改善情况在 2～ 8周最显著 ,对照组大鼠 (n =10 )的旋转行为无变化。结论 转基因的星形胶质细胞脑内移植后可短期改善PD大鼠的旋转行为 ,星形胶质细胞有可能作为有效的载体细胞。

Objective To evaluate the role of genetically modified astrocytes in the treatment of Parkinsonian rats. Methods Primary cultured astrocytes were genetically modified by plasmid pcDNA3 1/TH. Expression of TH was measured by immunocytochemistry staining and RT PCR. The TH modified cells were transplanted into PD rat models and the rotational behavior was observed. Results During the 12 weeks after transplantation, the rotational behavior of PD rats significantly improved( n =10, P <0 05), especially during 2～8 weeks, whereas the control group( n =10) had no change. Conclusions Intracerebral transplantation of genetically modified astrocytes significantly improve the rotational behavior of Parkinsonian rats. Astrocytes may be used as effective donor cells.