摘要
对虎杖(PolygonumcuspidatumSieb.etZucc.)中的白藜芦醇进行了提取分离和含量测定。考察了浸提温度、浸提液浓度、浸提时间和次数、料液比等5个因素对白藜芦醇提取的影响,确立了白藜芦醇最佳提取条件为:浸提液体积分数为95%,浸提温度60℃,料液比11∶1,回流提取3次,每次60min。采用中压硅胶柱层析法分离白藜芦醇,产物经重结晶后用高效液相色谱测定含量达99.28%。结果表明所建立的制备及分离纯化工艺可用于白藜芦醇的生产。
Extraction,separation,structure identification and content determination of resveratrol from Polygonum cuspidatum Sieb.et Zucc.had been carried out.Orthogonal experiment indicated that optimum extraction condition of resveratrol was:60 ℃,95% ethanol 1∶11(raw material∶ethanol),60 min and 3 times of extraction.Separation experiments with silica gel column under about 3 MPa pressure showed that ethyl acetate:petroleum ether was the ideal (eluant). After recrystallizing, resveratrol content was determined by HPLC to be as high as 99.28%.It can be (demonstrated) that this is a good technique used in resveratrol manufacture.
出处
《林产化学与工业》
EI
CAS
CSCD
2004年第1期39-42,共4页
Chemistry and Industry of Forest Products
关键词
白藜芦醇
提取
分离
中压柱层析
虎杖
含量测定
中药材
resveratrol
Polygonum cuspidatum Sieb.et Zucc.
orthogonal experiment
medium-pressure column chromatography
