短毛细管区带电泳快速测定增效联磺片中三组分含量

Rapid Determination of 3 Components in Sulfamethoxazole, Sulfadiazine and Trimethoprim Tablets by Short Capillary Zone Electrophoresis

目的:在短毛细管上应用毛细管区带电泳法快速测定增效联磺片中磺胺甲嗯唑(SMZ)、磺胺嘧啶(SD)及甲氧苄氨嘧啶(TMP)三组分的含量。方法:采用未涂层弹性石英毛细管(35cm×50 μm,有效长度20 cm),以咖啡因为内标(IS),检测波长214 nm(0.01 AUFS),电压10 kV,重力进样(10 cm×5 s),温度15℃,运行缓冲液为20 mmol·L-1硼砂溶液(磷酸调pH6.0)。每次电泳运行前用0.1 mol·L-1氢氧化钠溶液、水及缓冲溶液各冲洗毛细管3 min。结果:SMZ、SD、TMP的线性范围分别为0.0398-0.199,0.0399-0.200,0.0160-0.0800 mg·mL-1。平均回收率(n=9)分别为99.4%,99.0%,99.8%;RSD均小于1.5%。结论:该法简便快捷,准确可靠,灵敏度高。

Objective: To establish a rapid method using short capillary for the determination of 3 components sulfa methoxazole(SMZ) ,sulfadiazine(SD) ,and trimethoprim(TMP)in Sulfamethoxazole, Sulfadiazine and Trimethoprim tablets by capillary zone electrophoresis. Method:The separation was performed on a fused - silica capillary(35 cm ×50 μm,20 cm of effective length)and caffeine was used as the internal standard(IS). The detection was set at 214 nm(0. 01 AUFS)and the applied voltage was 10 kV. 10 cm ×5 s gravity injection was used and the temperature was 15 ℃.20 mmol·L-1 borate solution(pH was adjusted to 6. 0 with phosphoric acid)was used as running buffer. The capillary was rinsed with 0. 1 mol·L-1 sodium hydroxide solution, water, and running buffer for 3 min respectively before sample injection. Results: The linearity ranges of SMZ, SD, and TMP were 0.0398 - 0.199, 0.0399 -0.200,and 0.0160 - 0.0800 mg·mL-1 and the mean recoveries(n=9) were 99. 4% ,99.0% ,99. 8% , respectively,with RSDs were less than 1. 5%. Conclusion:The method is simple,rapid,accurate,reliable and sensitive for the determination of Sulfamethoxazole, Sulfadiazine and Trimethoprim tablets.