濒危植物元宝山冷杉的遗传多样性研究

The genetic diversity of the endangered plant Abies yuanbaoshanensis

元宝山冷杉(Abies yuanbaoshanensis)是仅分布于广西融水县元宝山的珍稀濒危物种。本研究采用AFLP分子标记分析了元宝山冷杉种群的遗传多样性。取元宝山冷杉种群43棵植株作为研究材料,4对引物组合用于AFLP扩增,共得到261条DNA扩增带。分析结果:元宝山冷杉种群的多态位点百分比率为50.96％,Nei's基因多样性为0.1510,Shannon多样性指数为0.1735。用NTSYS软件计算各个样品间的相似性系数,并用UPGMA法基于相似性系数进行了聚类分析。研究结果表明:元宝山冷杉种群的遗传多样性水平低,种群内个体间的相似性系数很大,无明显亚种群间的遗传分化。要保护或保存这个物种的遗传资源,建议应该选择种群内尽可能多的个体进行保护。

Abies yuanbaoshanensis (Pinaceae) is an endangered species endemic to Yuanbaoshan Mountain , Guangxi, China. Genetic diversity in 43 individuals of the speices was assessed using the amplified fragment length polymorphisms (AFLP) technique. Four primer combinations generated a total of 261 bands, of which 133 were polymorphic (50. 96%). The Nei's gene diversity of the population was 0. 1510, and Shannon's information index was 0. 1735. The results indicate that Abies yuanbaoshanensis has a low level of genetic diversity. This finding provides valuable information for formulating future conservation programs for the species.