无致病力青枯菌株对番茄青枯病的防治效果

Effects of Avirulent Strains of Ralstonia solanacearum on Tomato Bacterial Wilt

用紫外诱变法获得的青枯无致病力菌株ATm0 4 4和Asp0 6 1对致病菌没有直接的抑制作用 ;处理番茄后对青枯病产生抗性 ;两菌株可在番茄体内定殖并繁殖 ,移栽浸根是最佳的处理方法。盆栽试验结果表明 ,番茄经ATm0 4 4和Asp0 6 1处理后 ,分别较对照推迟 9和 7d发病 ,2 0d后的防效达 56 .7%和 53.4 %。田间小区试验结果表明 ,经ATm0 4 4和Asp0 6 1菌悬液浸根处理番茄 ,1 5d后对青枯病的防治效果分别为 53.8%和 37.7%。

Avirulent strains (ATm044 and Asp061) of Ralstonia solanacearum were obtained via UV mutagenesis. It was demonstrated that the strains could colonize tomato tissue and induce tomato resistance against bacterial wilt but no inhibition to virulent strains. The strains were also tested as a biocontrol agent against tomato bacterial wilt in greenhouse and field. Result showed that the disease was reduced by 56.7% and 53.4% in greenhouse 20d after treatment and 53.8% and 37.7% in field 15d after treatment by ATm044 and Asp061 respectively.