嗜线虫致病杆菌高产菌株的选育

Selection of High Antibiotics Production Strain from Xenorhabdus nematophila

用 4种诱变方法 (紫外线、氯化锂、60 Co γ射线、自身代谢产物 )对嗜线虫致病杆菌进行了诱变处理。结果表明 ,菌株对各种诱变剂均比较敏感 ,低剂量诱变和复合诱变处理的菌株正变率较高 ,其中氯化锂加紫外线复合诱变的累加处理效果最好 ,效价提高了 6 0 %～ 70 % ;连续5代传代培养 ,其产素能力基本稳定。

Xenorhabdus nematophila CB-6 strain was mutated by UV-ray, LiCl,^(60)Co-γ to increase production of antibiotic metabolites. Results showed that X. nematophila CB-6 strain was sensitive to all the mutagenic agents above. Low dose of mutagenic agent and multiple mutagenic agents was adapted to the bacteria. The strain mutated by the complex factors of UV-ray and LiCl was most effective for increasing antibiotics production. An effective strain with 60%-70% enhancement of antibiotics production compared with original strain was obtained.