实验性自身免疫性肝炎的研究

A preliminary study on experimental autoimmune hepatitis

本文通过 S-100肝抗原多次免疫同种 C57BL/6小鼠后,成功地建立了实验性自身免疫性肝炎的动物模型,并在此基础上进一步探讨了过继输注致敏脾淋巴细胞给正常同种小鼠后自身免疫性肝炎的发生情况.结果表明,导致过继输注后自身免疫性肝炎主要为 T 淋巴细胞,揭示了 T 淋巴细胞在自身免疫性肝炎发病中起着重要作用.另外,本文还就免疫抑制剂环孢素 A 对实验性自身免疫性肝炎的防治作用进行了探讨.组织学检查结果表明预防和治疗用药时均能减轻肝脏病理变化和/或减少自身免疫性肝炎的发生率.为临床治疗自身免疫性肝炎提供了实验室依据.

Experimental autoimmune hepatitis could be induced most effectively in C57BL/6 mice by immunization ith the syngeneic liver antigen S-100 in Complete Freud's Adjuvant.Histologicalchanges in liver were mostly marked -4 weeks after disease induction and could ever persist formore than 6 monthes.Adoptive transfer of hepatitis with rimed T lymphocytes but not Blymphocycs or macrophages was possible.Using a stronger immune inhibiting agent yclosporin A asfor both prevention and treatment of experimental hepatitis,it was noted that not only the incidenceof epatitis was dropped considerably,but also the severity of the disease was elleviated according tohistological indings.