人外周血淋巴细胞激活与CD2 mRNA 表达

Study on proliferation of HPBL and expression of CD2 mRNA

对 PHA、抗 CD3单克隆抗体 RW2—8C8、胸腺肽、抗 CD2与抗 CD3等对人 T 淋巴细胞的增殖现象及动力学进行了观察,除抗 CD3与抗 CD2单抗联合应用为抑制效应外,前三者均呈正效应且动力学完全一致.在此基础上,应用反义 CD2mRNA 探针检测了 mRNA 在激活24小时以后的变化,发现 T 细胞活化者均显示 mRNA 量呈上升;反之则不见变化或低于未激活 T 细胞对照组水平.本研究证明了小牛胸腺肽有激活 T 细胞和提高 mRNA 水平的作用,为首次报导.本文对上述在基因水平上观察的结果进行了讨论.

The effects of various stimuli on activation and proliferation of human peripheral blood lymphocytesHPBL)nd expression of CD2 mRNA were studied.The total RNA was extractedfrom the activatedHPBL and Northern blot ssay of these RNA samples were carried out with CD2 antisense RNAprobe prepared.It was shown that anti—CD3 Abs RW2-4B6 and RW2-8c8,PHA,and a calfthymic peptide preparation could all induce the activation and roliferation f HPBL and high levelexpression of CD2 mRNA.It was interresting to note that anti-CD3 McAb RW2-8c8 when usedtogether with antiT11_2 and anti-T11_3,inhibited the activation of HPBL and resulted in very low evelexpression of CD2 mRNA.The possible explaination of the phenomenon was discussed.