摘要
本实验对肿瘤细胞系的癌胚抗原(CEA)细胞粘附活性进行了研究。CEA 阳性肿瘤细胞系 LoVo及 HeLa 的单细胞悬液在37℃,RPMI1640全培养基中可以相互粘附而各自形成大小不等的细胞凝团。这种细胞间的粘附不仅可以被抗 CEA 多克隆抗体特异性阻断,而且受到外加 CEA 抗原的竞争抑制。细胞粘附试验也获得了同样结果。实验提示,CEA 为一粘附分子。
Cell adhesion activity of CEA on tumor cell lines was studied in the experiment.Single cell suspension of CEA positive ceils LoVo and HeLa aggregated respectively in RPMI1640 completemedium at 37℃ to form cell aggregates in different size.The cell aggregation could be specificly inhibited by anti-CEA polyclonal antibodies and purified CEA antigen.Similar results were obtained using cell adhesion assay.The experiment indicated that CEA is one of cell adhesion molecules.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1992年第2期90-94,共5页
Chinese Journal of Immunology
关键词
癌胚抗原
细胞凝集
细胞粘附
CEA
Cell adhesion
Adhesion molecules
Cell aggregation
