癌胚抗原细胞粘附作用的机理研究

Preliminary study on the mechanism of cell adhesion activity of CEA

已经证实癌胚抗原(CEA)为一粘附蛋白.本实验用 CEA 阳性细胞系 LoVo 细胞对 CEA 参与的细胞粘附机理进行了初步研究.电镜观察发现,LoVo 细胞通过细胞表面的微绒毛交叉接触而发生粘附,并可见桥粒及间隙连接形成.温度、NaN3、EDTA 二钠及细胞松驰素 B 均可明显抑制细胞间的相互粘附,表明 CEA 参与的细胞粘附过程是一个二价正离子参与的耗能过程,与细胞内微丝运动密切相关.文中还提出了 CEA 参与的细胞粘附作用的模式图.

Our previous experiment demonstrated that CEA is a cell adhesion molecule.The mechanism of cell adhesion activity of CEA was studied in this experiment using CEA positive cells line LoVo.Electron—micrographys study showed that LoVo cells adhered by cross contact of microvilli on the cell surface,and formation of gap junction and desomosomal junction were also observed.Cell adhesion activity of CEA was apperently influenced by emperature,NaN3,EDTA and cytochalasin B,showing that cell adhesion is an energy-consuming process depending on bivalent cation and movement of microfilament.A model of CEA on cell membrane was put forward.